Integrated label-free erbium-doped fiber laser biosensing system for detection of single cell Staphylococcus aureus

Liu, Jiandong, Liu, Bin, Liu, Juan, He, Xing-Dao, Yuan, Jinhui, Ghassemlooy, Fary, Torun, Hamdi, Fu, Yong Qing, Dai, Xuewu, Ng, Wai Pang, Binns, Richard and Wu, Qiang (2023) Integrated label-free erbium-doped fiber laser biosensing system for detection of single cell Staphylococcus aureus. Talanta. p. 124385. ISSN 0039-9140 (In Press)

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A critical challenge to realize ultra-high sensitivity with optical fiber interferometers for label free biosensing is to achieve high quality factors (Q-factor) in liquid. In this work a high Q-factor of 105, which significantly improves the detection resolution is described based on a structure of single mode -core-only -single mode fiber (SCS) with its multimode (or Mach-Zehnder) interference effect as a filter that is integrated into an erbium-doped fiber laser (EDFL) system for excitation. In the case study, the section of core-only fiber is functionalized with porcine immunoglobulin G (IgG) antibodies, which could selectively bind to bacterial pathogen of Staphylococcus aureus (S. aureus). The developed microfiber-based biosensing platform called SCS-based EDFL biosensors can effectively detect concentrations of S. aureus from 10 to 105 CFU/mL, with a responsivity of 42.6 p.m./(CFU/mL) for the wavelength shift of the measured spectrum. The limit of detection (LoD) is estimated as 7.3 CFU/mL based on the measurement of S. aureus with minimum concentration of 10 CFU/mL. In addition, when a lower concentration of 1 CFU/mL is applied to the biosensor, a wavelength shift of 0.12 nm is observed in 10% of samples (1/10), indicating actual LoD of 1 CFU/mL for the proposed biosensor. Attributed to its good sensitivity, stability, reproducibility and specificity, the proposed EDFL based biosensing platform has great potentials for diagnostics.

Item Type: Article
Additional Information: Funding information: This work was supported by the National Natural Science Foundation of China (NSFC) (11864025, 62175097, 62065013 and 62163029); Natural Science Foundation of Jiangxi Province (20212BAB202024 and 20192ACB20031); Royal Society International Exchanges 2020 Cost Share (NSFC) (IEC\NSFC\201015).
Uncontrolled Keywords: Staphylococcus aureus sensor, Foodborne pathogen, Fiber interferometer, Fiber laser biosensor
Subjects: F300 Physics
H600 Electronic and Electrical Engineering
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Rachel Branson
Date Deposited: 21 Feb 2023 10:36
Last Modified: 22 Feb 2023 15:51

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