Changes in the human plasma and urinary metabolome associated with acute dietary exposure to sucrose and the identification of potential biomarkers of sucrose intake

Beckmann, Manfred, Joosen, Annemiek M., Clarke, Michelle M., Mugridge, Owen, Frost, Gary, Engel, Barbara, Taillart, Kathleen, Lloyd, Amanda J., Draper, John and Lodge, John (2015) Changes in the human plasma and urinary metabolome associated with acute dietary exposure to sucrose and the identification of potential biomarkers of sucrose intake. Molecular Nutrition & Food Research, 60 (2). pp. 444-457. ISSN 1613-4125

[img] Text (Full text)
SBS metabolites accepted clean full paper with figs.docx - Accepted Version

Download (806kB)
[img]
Preview
Text (Full text)
SBS metabolites accepted clean full paper with figs.pdf - Accepted Version

Download (357kB) | Preview
Official URL: http://dx.doi.org/10.1002/mnfr.201500495

Abstract

Scope:
The intake of sucrose is of public health concern but limited information is available on the metabolic effects of short-term exposure. Our aim was to use metabolomics to investigate the metabolic impact of acute sucrose exposure.

Methods and results:
We performed a randomized, parallel, single-dose feeding study on healthy females (n = 90, aged 29.9 ± 4.7 years, BMI 23.3 ± 2.5 kg/m2) consuming either 0, 50, or 100 g sucrose in 500 mL water. Blood and urine samples were taken before and 24 h post sucrose intake. Urine and plasma samples underwent detailed metabolite profiling analysis using established protocols. Flow-injection electrospray MS fingerprinting analysis showed that 3 h after intake was the most informative time point in urine and plasma and out of 120 explanatory signals, highlighted 16 major metabolite signals in urine and 25 metabolite signals in plasma that were discriminatory and correlated with sucrose intake over time. The main confirmed metabolites positively correlated with intake were sucrose, fructose, and erythronic acid, while those negatively correlating with intake included fatty acids and derivatives, acyl-carnitines, and ketone bodies. GC-TOF-MS profiling analysis confirmed the fingerprinting data.

Conclusion:
Acute exposure to sucrose identified a number of metabolites correlated with sucrose intake and several compounds attributed to metabolic fasting.

Item Type: Article
Additional Information: Published online 15-9-2015 ahead of print
Uncontrolled Keywords: Biomarkers; Erythronic acid; Metabolism; Metabolomics; Sucrose
Subjects: B100 Anatomy, Physiology and Pathology
B400 Nutrition
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Paul Burns
Date Deposited: 30 Sep 2015 08:52
Last Modified: 24 May 2017 14:35
URI: http://nrl.northumbria.ac.uk/id/eprint/23909

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics


Policies: NRL Policies | NRL University Deposit Policy | NRL Deposit Licence