Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri

Yi, Yue, Dolfing, Jan, Jin, Ge, Fang, XiaoYu, Han, WenHao, Liu, LaiYan, Tang, YueQin and Cheng, Lei (2023) Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri. Water Research, 232. p. 119664. ISSN 0043-1354

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Ammonia is a ubiquitous potential inhibitor of anaerobic digestion processes, mainly exhibiting inhibition towards methanogenic activity. However, knowledge as to how ammonia affects the methanogens is still limited. In this study, we cultured a multitrophic methanogen, Methanosarcina barkeri DSM 800, with acetate, H2/CO2, and methanol to evaluate the influence of ammonia on different methanogenic pathways. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. Theoretical maximum NH3 tolerances of M. barkeri fed with acetate, H2/CO2, and methanol were calculated to be 39.1 ± 9.0, 104.3 ± 7.4, and 85.7 ± 1.0 mg/L, respectively. The order of the ΔG range of M. barkeri under three methanogenic pathways reflected the order of ammonia tolerance of M. barkeri. Our results provide insights into the role of the thermodynamic potential of methanogenesis on the tolerance of ammonia stress; and shed light on the mechanism of ammonia inhibition on anaerobic digestion.

Item Type: Article
Additional Information: Funding information: This study was supported by National Natural Science Foundation of China (no. 92051108), Agricultural Science and Technology Innovation Project of the Chinese Academy of Agriculture Science (no. CAAS-ASTIP-2016-BIOMA), the Central Public-interest Scientific Institution Basal Research Fund (Y2021PT02, Y2021XK06, 2013ZL001), Hainan Science and Technology Program (ZDYF2021XDNY300).
Uncontrolled Keywords: Ammonia tolerance, Methanosarcina barkeri, Gibbs free energy, Thermodynamic restriction
Subjects: D700 Agricultural Sciences
H300 Mechanical Engineering
H800 Chemical, Process and Energy Engineering
Department: Faculties > Engineering and Environment > Mechanical and Construction Engineering
Depositing User: Elena Carlaw
Date Deposited: 28 Feb 2023 09:04
Last Modified: 24 Jan 2024 03:30

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